Correction to “Trehalose-Based Polyethers for Cryopreservation and 3D Cell Scaffolds”
نویسندگان
چکیده
منابع مشابه
Cryopreservation of hepatocyte (HepG2) cell monolayers: impact of trehalose.
A simple method to cryogenically preserve hepatocyte monolayers is currently not available but such a technique would facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. We investigated the effect of trehalose and dimethyl sulfoxide (Me2SO) in cryopreservation of human hepatocellular carcinoma (HepG2) cells in suspension and monola...
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متن کاملPredehydration and Ice Seeding in the Presence of Trehalose Enable Cell Cryopreservation
Conventional approaches for cell cryopreservation require the use of toxic membrane-penetrating cryoprotective agents (pCPA), which limits the clinical application of cryopreserved cells. Here, we show intentionally induced ice formation at a high subzero temperature (> -10 °C) during cryopreservation, which is often referred to as ice seeding, could result in significant cell injury in the abs...
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There are numerous approaches for producing natural and synthetic 3D scaffolds that support the proliferation of mammalian cells. 3D scaffolds better represent the natural cellular microenvironment and have many potential applications in vitro and in vivo. Here, we demonstrate that 3D cellulose scaffolds produced by decellularizing apple hypanthium tissue can be employed for in vitro 3D culture...
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The biggest challenge of designing and implementing an in vitro study is developing a microenvironment that most closely represents the interactions observed in vivo. Decellularization of tissues and organs has been shown to be an effective method for the removal of potentially immunogenic constituents while preserving essential growth factors and extracellular matrix (ECM ) proteins necessary ...
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ژورنال
عنوان ژورنال: Biomacromolecules
سال: 2021
ISSN: 1525-7797,1526-4602
DOI: 10.1021/acs.biomac.1c00671